ARF1 Monoclonal antibody

ARF1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C10E3

Cat no : 68069-1-Ig

Synonyms

ADP ribosylation factor 1, ARF1



Tested Applications

Positive WB detected inA431 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, rabbit brain tissue, mouse brain tissue, rat brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68069-1-Ig targets ARF1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARF1 fusion protein Ag28021
Full Name ADP-ribosylation factor 1
Calculated molecular weight 21 kDa
Observed molecular weight18-21 kDa
GenBank accession numberBC009247
Gene symbol ARF1
Gene ID (NCBI) 375
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562).

Protocols

Product Specific Protocols
WB protocol for ARF1 antibody 68069-1-IgDownload protocol
IF protocol for ARF1 antibody 68069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols