Validation Data Gallery
|Positive WB detected in
|DU 145 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, SKOV-3 cells, MDA -MB-231 cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human brain tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
20226-1-AP targets ARF1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|ADP-ribosylation factor 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.
|Product Specific Protocols
|WB protocol for ARF1 antibody 20226-1-AP
|IHC protocol for ARF1 antibody 20226-1-AP
|IF protocol for ARF1 antibody 20226-1-AP
|IP protocol for ARF1 antibody 20226-1-AP
|Click here to view our Standard Protocols
Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.
Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation
ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling
Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.
J Cell Sci
The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response.
In vivo monitoring of the recruitment and activation of AP-1 by Arf1.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Cristian (Verified Customer) (04-26-2019)
Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 20226-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)