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ARF1 Polyclonal antibody

ARF1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20226-1-AP

Synonyms

ADP ribosylation factor 1, ARF1



Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, SKOV-3 cells, MDA -MB-231 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human brain tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20226-1-AP targets ARF1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ADP-ribosylation factor 1
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberNM_001658
Gene symbol ARF1
Gene ID (NCBI) 375
RRIDAB_2878659
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.

Protocols

Product Specific Protocols
WB protocol for ARF1 antibody 20226-1-APDownload protocol
IHC protocol for ARF1 antibody 20226-1-APDownload protocol
IF protocol for ARF1 antibody 20226-1-APDownload protocol
IP protocol for ARF1 antibody 20226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Immunol

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Authors - Miwa Sasai
human,mouseWB

Nat Commun

Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation

Authors - Mutian Jia
humanWB

Nat Commun

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling

Authors - Maximilian Hirschenberger
humanWB

PLoS Pathog

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Authors - Tao Wang
humanWB,IF

J Cell Sci

The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response.

Authors - Yuanyuan Su
humanWB

Sci Rep

In vivo monitoring of the recruitment and activation of AP-1 by Arf1.

Authors - Etienne Sauvageau

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Cristian (Verified Customer) (04-26-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 20226-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa
ARF1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:20226-1-AP)