Tested Applications
| Positive WB detected in | DU 145 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, SKOV-3 cells, MDA -MB-231 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human brain tissue, human liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20226-1-AP targets ARF1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ADP-ribosylation factor 1 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 18-21 kDa |
| GenBank Accession Number | NM_001658 |
| Gene Symbol | ARF1 |
| Gene ID (NCBI) | 375 |
| RRID | AB_2878659 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P84077 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ARF1 antibody 20226-1-AP | Download protocol |
| IF protocol for ARF1 antibody 20226-1-AP | Download protocol |
| IHC protocol for ARF1 antibody 20226-1-AP | Download protocol |
| IP protocol for ARF1 antibody 20226-1-AP | Download protocol |
| WB protocol for ARF1 antibody 20226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARF1 antibody (Cat# 20226-1-AP) has 11 citations published in journals including Cell Metabolism, Nature Immunology, Nature Communications, Advanced Science, EMBO Reports, Scientific Reports, PLoS Pathogens, Journal of Biological Chemistry, and Journal of Cell Science. Research fields span interferon signaling, autophagy, cGAS-STING pathway, stroke/neuroprotection, oocyte meiosis, viral infection, ERAD pathway, atherosclerosis, and classical secretory pathway regulation.
| Species | Application | Title |
|---|---|---|
Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation | ||
Nat Immunol Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense. | ||
Nat Commun ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling | ||
Nat Commun Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation | ||
Adv Sci (Weinh) Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis
| ||
EMBO Rep Distinct pathogenic mutations in ARF1 allow dissection of its dual role in cGAS-STING signalling |
Reviews
What customers say
One user review is available for this product. Cristian Pocognoni (2019) rated it 4 out of 5 stars after using the ARF1 antibody for immunofluorescence on PFA-fixed HeLa cells at a 1:500 dilution, paired with an Alexa Fluor 594-conjugated goat anti-rabbit IgG secondary antibody. The reviewer shared a representative image showing clear staining results, indicating satisfactory performance for IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cristian (Verified Customer) (04-26-2019) | Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 20226-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)
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