• Featured Product
  • KD/KO Validated

ARF1 Polyclonal antibody

ARF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20226-1-AP

Synonyms

ADP ribosylation factor 1, ARF1



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human brain tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

20226-1-AP targets ARF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ADP-ribosylation factor 1
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberNM_001658
Gene symbol ARF1
Gene ID (NCBI) 375
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.

Protocols

Product Specific Protocols
WB protocol for ARF1 antibody 20226-1-APDownload protocol
IHC protocol for ARF1 antibody 20226-1-APDownload protocol
IF protocol for ARF1 antibody 20226-1-APDownload protocol
IP protocol for ARF1 antibody 20226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

In vivo monitoring of the recruitment and activation of AP-1 by Arf1.

Authors - Etienne Sauvageau
humanWB

PLoS Pathog

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Authors - Tao Wang
mouseWB

Nat Immunol

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Authors - Miwa Sasai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Cristian (Verified Customer) (04-26-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 20226-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa
ARF1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:20226-1-AP)