Validation Data Gallery
|Positive WB detected in||DU 145 cells, HepG2 cells, mouse brain tissue, HEK-293 cells, HeLa cells, SKOV-3 cells, MDA -MB-231 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human brain tissue, human colon cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20226-1-AP targets ARF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||ADP-ribosylation factor 1|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||18-21 kDa|
|GenBank accession number||NM_001658|
|Gene ID (NCBI)||375|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.
|Product Specific Protocols|
|WB protocol for ARF1 antibody 20226-1-AP||Download protocol|
|IHC protocol for ARF1 antibody 20226-1-AP||Download protocol|
|IF protocol for ARF1 antibody 20226-1-AP||Download protocol|
|IP protocol for ARF1 antibody 20226-1-AP||Download protocol|
|Click here to view our Standard Protocols|
In vivo monitoring of the recruitment and activation of AP-1 by Arf1.
J Cell Sci
The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response.
Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.
Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Cristian (Verified Customer) (04-26-2019)
Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 20226-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)