ARF5 Monoclonal antibody

ARF5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B3B1

Cat no : 67279-1-Ig

Synonyms

ADP ribosylation factor 5, ARF5

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Validation Data Gallery

• 

  WB analysis using 67279-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67279-1-Ig (ARF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67279-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67279-1-Ig (ARF5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67279-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67279-1-Ig (ARF5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67279-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67279-1-Ig (ARF5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67279-1-Ig targets ARF5 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARF5 fusion protein Ag7849
• Full Name: ADP-ribosylation factor 5
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC003043
• Gene Symbol: ARF5
• Gene ID (NCBI): 381
• RRID: AB_2882547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.