Arginase-2 Polyclonal antibody, PBS Only

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• Arginase-2 Polyclonal antibody, PBS Only (14825-1-PBS)

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  WB analysis of Caco-2 using 14825-1-AP (same clone as 14825-1-PBS)

  WB result of ARG2 antibody (14825-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arginase-2 transfected Caco-2 cells. This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  WB analysis using 14825-1-AP (same clone as 14825-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 14825-1-AP (Arginase-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  WB analysis using 14825-1-AP (same clone as 14825-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14825-1-AP (Arginase-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14825-1-AP (ARG2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Arginase-2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14825-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ARG2, ARG 2, EC:3.5.3.1, Arginase-2, mitochondrial, arginase, type II

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Product Information

14825-1-PBS targets Arginase-2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6609

  Product name: Recombinant human ARG2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-354 aa of BC001350

  Sequence: MSLRGSLSRLLQTRVHSILKKSVHSVAVIGAPFSQGQKRKGVEHGPAAIREAGLMKRLSSLGCHLKDFGDLSFTPVPKDDLYNNLIVNPRSVGLANQELAEVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVDAHADINTPLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSASIVYIGLRDVDPPEHFILKNYDIQYFSMRDIDRLGIQKVMERTFDLLIGKRQRPIHLSFDIDAFDPTLAPATGTPVVGGLTYREGMYIAEEIHNTGLLSALDLVEVNPQLATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQARVRI

• Full Name: arginase, type II
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC001350
• Gene Symbol: ARG2
• Gene ID (NCBI): 384
• RRID: AB_2058977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78540
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)