ARHGEF4 Polyclonal antibody, PBS Only

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• ARHGEF4 Polyclonal antibody, PBS Only (55213-1-PBS)

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  WB analysis of mouse brain using 55213-1-AP (same clone as 55213-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

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  WB analysis of multi-cells/tissue using 55213-1-AP (same clone as 55213-1-PBS)

  WB result of 55213-1-AP (ARHGEF4 Antibody). This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 55213-1-AP (same clone as 55213-1-PBS)

  IP result of anti-ARHGEF4 (IP:55213-1-AP, 4ug; Detection:55213-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55213-1-AP (same clone as 55213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55213-1-AP (same clone as 55213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55213-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ARHGEF4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 55213-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

55213-1-PBS targets ARHGEF4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Rho guanine nucleotide exchange factor (GEF) 4
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_015320
• Gene Symbol: ARHGEF4
• Gene ID (NCBI): 50649
• RRID: AB_2721023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NR80
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.