ARL8A/ARL8B Monoclonal antibody

ARL8A/ARL8B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F4C3

Cat no : 68186-1-Ig

Synonyms

ARL10B, ARL8A, FLJ45195, GIE2

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Validation Data Gallery

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  WB analysis using 68186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 68186-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68186-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rabbit brain tissue, LNCaP cells, human placenta tissue, HeLa cells, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68186-1-Ig targets ARL8A/ARL8B in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARL8A/ARL8B fusion protein Ag10758
• Full Name: ADP-ribosylation factor-like 8A
• Calculated Molecular Weight: 186 aa, 21 kDa
• Observed Molecular Weight: 18 kDa, 21 kDa
• GenBank Accession Number: BC015408
• Gene Symbol: ARL8A
• Gene ID (NCBI): 127829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A/ARL8B can be recognized by this antibody.