ARRDC1 Recombinant antibody

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  WB analysis using 84874-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84874-5-RR (ARRDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ARRDC1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84874-5-RR

Clone No.242396A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242396A5, Alpha-arrestin 1, arrestin domain containing 1, Arrestin domain-containing protein 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HL-60 cells, K-562 cells, HeLa cells, HepG2 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84874-5-RR targets ARRDC1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARRDC1 fusion protein Ag15341
• Full Name: arrestin domain containing 1
• Calculated Molecular Weight: 433 aa, 46 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC032346
• Gene Symbol: ARRDC1
• Gene ID (NCBI): 92714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N5I2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARRDC1, or Arrestin-Domain Containing Protein 1, is a protein that plays a significant role in the formation and release of extracellular vesicles, specifically a subtype known as ARMMs (Arrestin-Domain Containing Protein 1-mediated microvesicles). ARRDC1 is localized to the cytosolic side of the plasma membrane and recruits the ESCRT-I complex protein TSG101 to initiate outward membrane budding. This protein is also involved in the regulation of protein cargo and release of extracellular vesicles, including both exosomes and ectosomes. The absence of ARRDC1 can lead to changes in the protein cargo within these vesicles, indicating its importance in the sorting and packaging process.