CoraLite® Plus 488-conjugated ASCC2 Polyclonal antibody

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  IF Staining of HepG2 using CL488-11529

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ASCC2 antibody (CL488-11529) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HepG2 using CL488-11529

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Ascc2 Polyclonal Antibody (CL488-11529)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

ASCC2 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-11529

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

ASC-1 complex subunit p100, ASC1P100, RQT3

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11529 targets ASCC2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2100

  Product name: Recombinant human ASCC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 408-757 aa of BC025368

  Sequence: RRKATDAKDPSVIEEPNGEPNGVTVTAEAVSQASSHPENSEEEECMGAAAAVGPAMCGVELDSLISQVKDLLPDLGEGFILACLEYYHYDPEQVINNILEERLAPTLSQLDRNLDREMKPDPTPLLTSRHNVFQNDEFDVFSRDSVDLSRVHKGKSTRKEENTRSLLNDKRAVAAQRQRYEQYSVVVEEVPLQPGESLPYHSVYYEDEYDDTYDGNQVGANDADSDDELISRRPFTIPQVLRTKVPREGQEEDDDDEEDDADEEAPKPDHFVQDPAVLREKAEARRMAFLAKKGYRHDSSTAVAGSPRGHGQSRETTQERRKKEANKATRANHNRRTMADRKRSKGMIPS

• Full Name: activating signal cointegrator 1 complex subunit 2
• Calculated Molecular Weight: 757 aa, 86 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC025368
• Gene Symbol: ASCC2
• Gene ID (NCBI): 84164
• RRID: AB_3672501
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H1I8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.