ASGR2 Polyclonal antibody, PBS Only

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• ASGR2 Polyclonal antibody, PBS Only (11501-2-PBS)

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  WB analysis of human liver using 11501-2-AP (same clone as 11501-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11501-2-AP (ASGR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11501-2-AP (same clone as 11501-2-PBS)

  WB result of ASGR2 antibody (11501-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR2 transfected HepG2 cells. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 11501-2-AP (same clone as 11501-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11501-2-AP (ASGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 11501-2-AP (same clone as 11501-2-PBS)

  IP result of anti-ASGR2 (IP:11501-2-AP, 4ug; Detection:11501-2-AP 1:300) with L02 cells lysate 1800ug. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11501-2-AP (same clone as 11501-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11501-2-AP (same clone as 11501-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 11501-2-AP (same clone as 11501-2-PBS)

  1x10^6 HepG2 cells were surface stained with 0.25 ug ASGR2 Polyclonal antibody (11501-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were not fixed. This data was developed using the same antibody clone with 11501-2-PBS in a different storage buffer formulation.

ASGR2 Polyclonal Antibody for WB, IHC, FC, IP, Indirect ELISA

Cat No. 11501-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, IP, Indirect ELISA

Synonyms

ASGP R, ASGP R 2, ASGPR 2, ASGP-R 2, asialoglycoprotein receptor 2

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Product Information

11501-2-PBS targets ASGR2 in WB, IHC, FC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2037

  Product name: Recombinant human ASGR2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-287 aa of BC017251

  Sequence: MAKDFQDIQQLSSEENDHPFHQGPPPAQPLAQRLCSMVCFSLLALSFNILLLVVICVTGSQSAQLQAELRSLKEAFSNFSSSTLTEVQAISTHGGSVGDKITSLGAKLEKQQQDLKADHDALLFHLKHFPVDLRFVACQMELLHSNGSQRTCCPVNWVEHQGSCYWFSHSGKAWAEAEKYCQLENAHLVVINSWEEQKFIVQHTNPFNTWIGLTDSDGSWKWVDGTDYRHNYKNWAVTQPDNWHGHELGGSEDCVEVQPDGRWNDDFCLQVYRWVCEKRRNATGEVA

• Full Name: asialoglycoprotein receptor 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 35 kDa, 40-50 kDa
• GenBank Accession Number: BC017251
• Gene Symbol: ASGR2
• Gene ID (NCBI): 433
• RRID: AB_2059814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07307
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.