ASK1 Monoclonal antibody

ASK1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C3A10

Cat no : 67072-1-Ig

Synonyms

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5



Tested Applications

Positive WB detected inHSC-T6 cells, HEK-293 cells, Jurkat cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue, mouse heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67072-1-Ig targets ASK1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASK1 fusion protein Ag5818
Full Name mitogen-activated protein kinase kinase kinase 5
Calculated molecular weight 155 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC054503
Gene symbol MAP3K5
Gene ID (NCBI) 4217
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for ASK1 antibody 67072-1-IgDownload protocol
IHC protocol for ASK1 antibody 67072-1-IgDownload protocol
IF protocol for ASK1 antibody 67072-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols