CoraLite® Plus 488-conjugated ASK1 Monoclonal antibody

ASK1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3A10

Cat no : CL488-67072

Synonyms

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5



Tested Applications

Positive IF detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67072 targets ASK1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASK1 fusion protein Ag5818
Full Name mitogen-activated protein kinase kinase kinase 5
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC054503
Gene Symbol MAP3K5
Gene ID (NCBI) 4217
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ASK1 antibody CL488-67072Download protocol
FC protocol for CL Plus 488 ASK1 antibody CL488-67072Download protocol
Standard Protocols
Click here to view our Standard Protocols