CoraLite® Plus 488-conjugated ASL Monoclonal antibody

ASL Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G9H3

Cat no : CL488-67692

Synonyms

argininosuccinate lyase, Arginosuccinase, ASAL, ASL



Tested Applications

Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-67692 targets ASL in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ASL fusion protein Ag30517
Full Name argininosuccinate lyase
Calculated molecular weight 55 kDa
Observed molecular weight50 kDa
GenBank accession numberBC008195
Gene symbol ASL
Gene ID (NCBI) 435
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 ASL antibody CL488-67692Download protocol
Standard Protocols
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