ATP5S Polyclonal antibody, PBS Only

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• ATP5S Polyclonal antibody, PBS Only (16335-1-PBS)

• 

  WB analysis using 16335-1-AP (same clone as 16335-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16335-1-AP (ATP5S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16335-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 16335-1-AP (same clone as 16335-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ATP5S antibody (16335-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16335-1-PBS in a different storage buffer formulation.

ATP5S Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 16335-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ATP synthase-coupling factor B, ATPW, Distal membrane arm assembly complex 2-like protein, DMAC2L, FB

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Product Information

16335-1-PBS targets ATP5S in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9486

  Product name: Recombinant human ATP5S protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-127 aa of BC011549

  Sequence: MCCAVSEQRLTCADQMMLFGKISQQLCGVKKLPWSCDSRYFWGWLNAVFNKVDYDRIRDVGPDRAASEWLLRCGAMVRYHGQERWQKDYNHLPTGPLDKYKIQAIDATDSCIMSIGFDHMETSNICC

• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit s (factor B)
• Calculated Molecular Weight: 127aa,15 kDa; 215aa,25 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC011549
• Gene Symbol: ATP5S
• Gene ID (NCBI): 27109
• RRID: AB_3741884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99766
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DMAC2L also known as ATP5S, belongs to the ATP synthase family, which is a group of enzymes that play a crucial role in cellular energy production. ATP synthase is the central enzyme in oxidative phosphorylation, responsible for the production of most of the ATP in mammalian organisms. ATP5S is the S subunit (also known as factor B) of mitochondrial FO complex of ATP synthase. It is an essential component of the H+ channel of the FO complex (PMID:7706317, 6143319).