ATP6V0A4 Polyclonal antibody, PBS Only

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• ATP6V0A4 Polyclonal antibody, PBS Only (21570-1-PBS)

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  WB analysis using 21570-1-AP (same clone as 21570-1-PBS)

  Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21570-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 21570-1-AP (same clone as 21570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21570-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 21570-1-AP (same clone as 21570-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ATP6V0A4 antibody (21570-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21570-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ATP6V0A4 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 21570-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

A4, ATP6N1B, ATP6N2, RDRTA2, RTA1C

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Product Information

21570-1-PBS targets ATP6V0A4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16095

  Product name: Recombinant human ATP6V0A4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 190-375 aa of BC109305

  Sequence: ERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPERAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAG

• Full Name: ATPase, H+ transporting, lysosomal V0 subunit a4
• Calculated Molecular Weight: 840 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC109305
• Gene Symbol: ATP6V0A4
• Gene ID (NCBI): 50617
• RRID: AB_2918072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HBG4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness. This antibody was generated against the internal region of human ATP6V0A4 and is predicted to detect the a4 isoform only.