ATP6V0D1 Monoclonal antibody

ATP6V0D1 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G7A12

Cat no : 68506-1-Ig

Synonyms

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

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Validation Data Gallery

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  WB analysis using 68506-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 68506-1-Ig

  IP result of anti-ATP6V0D1 (IP:68506-1-Ig, 4ug; Detection:68506-1-Ig 1:3000) with HeLa cells lysate 1520 ug.

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  IHC staining of mouse kidney using 68506-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 68506-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68506-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (68506-1-Ig, Clone: 1G7A12 ) at dilution of 1:2400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, zebrafish tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:1200-1:4800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68506-1-Ig targets ATP6V0D1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP6V0D1 fusion protein Ag13012
• Full Name: ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
• Calculated Molecular Weight: 351 aa, 40 kDa
• Observed Molecular Weight: 37-41 kDa
• GenBank Accession Number: BC008861
• Gene Symbol: ATP6V0D1
• Gene ID (NCBI): 9114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.