• Featured Product
  • KD/KO Validated

AZI1 Polyclonal antibody

AZI1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 25735-1-AP

Synonyms

5 azacytidine induced 1, AZ1, AZI1, Centrosomal protein of 131 kDa, Cep131, KIAA1118



Tested Applications

Positive WB detected inmouse testis tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25735-1-AP is AZI1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AZI1 fusion protein Ag22699
Full Name 5-azacytidine induced 1
Calculated Molecular Weight 1083 aa, 122 kDa
Observed Molecular Weight 131 kDa
GenBank Accession NumberBC136578
Gene Symbol AZI1
Gene ID (NCBI) 22994
RRIDAB_2880216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

Protocols

Product Specific Protocols
WB protocol for AZI1 antibody 25735-1-APDownload protocol
IF protocol for AZI1 antibody 25735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
humanWB,IF,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
  • KD Validated
human,mouseIF

Elife

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis

Authors - Emma A Hall
humanIF

PLoS Biol

Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.

Authors - Özge Z Aydin
humanWB

Front Oncol

USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.

Authors - Catherine M Nickson
  • KD Validated
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jay (Verified Customer) (10-15-2019)

Methanol fixation,Immunostaining with Azi1(red), centrosome marker gamma-tubulin(green) and DAPI(blue) for nucleus.First time to show IF result using mouse NIH3T3 cell line.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse NIH3T3
AZI1 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 (Cat no:25735-1-AP)