Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25735-1-AP targets AZI1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22699 Product name: Recombinant human AZI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 730-1080 aa of BC136578 Sequence: HEAELLQSDERASQRCLRQAEELREQLEREKEALGQQERERARQRFQQHLEQEQWALQQQRQRLYSEVAEERERLGQQAARQRAELEELRQQLEESSSALTRALRAEFEKGREEQERRHQMELNTLKQQLELERQAWEAGRTRKEEAWLLNREQELREEIRKGRDKEIELVIHRLEADMALAKEESEKAAESRIKRLRDKYEAELSELEQSERKLQERCSELKGQLGEAEGENLRLQGLVRQKERALEDAQAVNEQLSSERSNLAQVIRQEFEDRLAASEEETRQAKAELATLQARQQLELEEVHRRVKTALARKEEAVSSLRTQHEAAVKRADHLEELLEQHRRPTPSTK Predict reactive species |
| Full Name | 5-azacytidine induced 1 |
| Calculated Molecular Weight | 1083 aa, 122 kDa |
| Observed Molecular Weight | 131 kDa |
| GenBank Accession Number | BC136578 |
| Gene Symbol | AZI1 |
| Gene ID (NCBI) | 22994 |
| RRID | AB_2880216 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPN4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AZI1 antibody 25735-1-AP | Download protocol |
| WB protocol for AZI1 antibody 25735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AZI1 Polyclonal antibody (Cat# 25735-1-AP) has 17 citations across journals including Nature Communications, Cell Reports, Developmental Cell, PLoS Biology, eLife, BMC Biology, Cells, Communications Biology, RNA, Journal of Cell Science, Journal of Cell Physiology, Frontiers in Oncology, Molecular Human Reproduction, and Cell Molecular Biology Letters. Research fields span centrosome/centriole biology, ciliogenesis, centriolar satellites, ubiquitin E3 ligase substrates, atherosclerosis, breast carcinogenesis, RNA splicing, meiosis, and pancreatic cancer.
| Species | Application | Title |
|---|---|---|
Nat Commun USP9X regulates centrosome duplication and promotes breast carcinogenesis.
| ||
Nat Commun Lipid droplets sequester palmitic acid to disrupt endothelial ciliation and exacerbate atherosclerosis in male mice | ||
Cell Mol Biol Lett CCHCR1 links P-body proteins to the centrosome and is required for ciliogenesis through interacting with OFD1 and PCM1 | ||
Dev Cell Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation.
| ||
Cell Rep Coiled-coil-domain-mediated TRIM E3 protein condensation modulates enzymatic activity and functional specificity |
Reviews
What customers say
The single review for this antibody is a 5-star rating from an immunofluorescence experiment on mouse NIH3T3 cells. The user employed methanol fixation and stained with Azi1 (red), a gamma-tubulin centrosome marker (green), and DAPI (blue) for nuclear counterstaining. They noted it was their first time obtaining an IF result with this cell line, and the antibody performed well at a 1:100 dilution. Overall, the feedback is positive, confirming reliable staining for Azi1 detection in mouse cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jay (Verified Customer) (10-15-2019) | Methanol fixation,Immunostaining with Azi1(red), centrosome marker gamma-tubulin(green) and DAPI(blue) for nucleus.First time to show IF result using mouse NIH3T3 cell line.
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