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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AZI1 Polyclonal antibody

AZI1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25735

Synonyms

5 azacytidine induced 1, AZ1, AZI1, Centrosomal protein of 131 kDa, Cep131, KIAA1118

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated AZI1 Polyclonal anti (CL488-25735)

    FC experiment of HeLa using CL488-25735

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AZI1 (CL488-25735) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-25735 is AZI1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AZI1 fusion protein Ag22699
Full Name 5-azacytidine induced 1
Calculated Molecular Weight 1083 aa, 122 kDa
Observed Molecular Weight 131 kDa
GenBank Accession NumberBC136578
Gene Symbol AZI1
Gene ID (NCBI) 22994
RRIDAB_2919204
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 AZI1 antibody CL488-25735Download protocol
Standard Protocols
Click here to view our Standard Protocols