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CoraLite®555-conjugated AlaRS Monoclonal antibody

AlaRS Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1C8H3

Cat no : CL555-67909

Synonyms

AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated AlaRS Monoclonal antibody (CL555-67909)

    IF Staining of HeLa using CL555-67909

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 AlaRS antibody (CL555-67909, Clone: 1C8H3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of K-562 cells using CoraLite®555-conjugated AlaRS Monoclonal antibody (CL555-67909)

    FC experiment of K-562 using CL555-67909

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human AlaRS (CL555-67909, Clone:1C8H3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67909 targets AlaRS in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AlaRS fusion protein Ag11151
Full Name alanyl-tRNA synthetase
Calculated Molecular Weight 968 aa, 107 kDa
Observed Molecular Weight107 kDa
GenBank Accession NumberBC011451
Gene Symbol AARS
Gene ID (NCBI) 16
RRIDAB_2919706
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

Protocols

Product Specific Protocols
IF protocol for CL555 AlaRS antibody CL555-67909Download protocol
FC protocol for CL555 AlaRS antibody CL555-67909Download protocol
Standard Protocols
Click here to view our Standard Protocols