CoraLite®555-conjugated AlaRS Monoclonal antibody
AlaRS Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
IF, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
1C8H3
Cat no : CL555-67909
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HeLa cells |
Positive FC detected in | K-562 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL555-67909 targets AlaRS in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AlaRS fusion protein Ag11151 |
Full Name | alanyl-tRNA synthetase |
Calculated molecular weight | 968 aa, 107 kDa |
Observed molecular weight | 107 kDa |
GenBank accession number | BC011451 |
Gene symbol | AARS |
Gene ID (NCBI) | 16 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).
Protocols
Product Specific Protocols | |
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IF protocol for CL555 AlaRS antibody CL555-67909 | Download protocol |
FC protocol for CL555 AlaRS antibody CL555-67909 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |