Alpha 1B-Glycoprotein Monoclonal antibody

Alpha 1B-Glycoprotein Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4D4E5

Cat no : 66260-1-Ig

Synonyms

A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, GAB, HYST2477



Tested Applications

Positive WB detected inhuman plasma tissue, human heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66260-1-Ig targets Alpha 1B-Glycoprotein in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha 1B-Glycoprotein fusion protein Ag5414
Full Name alpha-1-B glycoprotein
Calculated molecular weight 54 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC035719
Gene symbol A1BG
Gene ID (NCBI) 1
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

Protocols

Product Specific Protocols
WB protocol for Alpha 1B-Glycoprotein antibody 66260-1-IgDownload protocol
IHC protocol for Alpha 1B-Glycoprotein antibody 66260-1-IgDownload protocol
IF protocol for Alpha 1B-Glycoprotein antibody 66260-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols