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Amphiphysin Polyclonal antibody, PBS Only (13379-1-PBS)

Filter: AllWBIPIHC

WB analysis of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 13379-1-AP (same clone as 13379-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)
IP result of anti-Amphiphysin (IP:13379-1-AP, 4ug; Detection:13379-1-AP 1:1500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 13379-1-AP (same clone as 13379-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 13379-1-AP (same clone as 13379-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 13379-1-AP (same clone as 13379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 13379-1-AP (same clone as 13379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Amphiphysin Polyclonal antibody, PBS Only

Name: Amphiphysin Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13379-1-PBS

Amphiphysin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 13379-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

13379-1-PBS targets Amphiphysin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4204 Product name: Recombinant human AMPH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 346-695 aa of BC034376 Sequence: KKEETLLDLDFDPFKPEVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQPQDTSLFTMQTDQSMICNLAESEQAPPTEPKAEEPLAAVTPAVGLDLGMDTRAEEPVEEAVIIPGADADAAVGTLVSAAEGAPGEEAEAETATVPAGEGVSLEEAKIGTETTEGAESAQPEAEELEATVPQEKVIPSVVIEPASNHEEEGENEITIGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPAMGAADQLASAREASQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLD Predict reactive species
Full Name	amphiphysin
Calculated Molecular Weight	695 aa, 76 kDa
Observed Molecular Weight	115-125 kDa
GenBank Accession Number	BC034376
Gene Symbol	AMPH
Gene ID (NCBI)	273
RRID	AB_2226789
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49418
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Amphiphysin (AMPH), a synaptic vesicle-associated protein that is highly concentrated in nerve terminal, is an autoantigen of Stiff-Man syndrome with breast cancer. Amphiphysin contains a N-terminal BAR (Bin1/Amphiphysin/Rv167) domain followed by an endocytosis domain, and a C-terminal SH3 domain. Amphiphysin has been proposed to function as a linker between the clathrin coat and dynamin in the endocytosis of synaptic vesicles. It may also participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. (PMID: 8552632; 8245793; 10559861)