Annexin A3 Monoclonal antibody

Annexin A3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11E4

Cat no : 66405-1-Ig

Synonyms

35 alpha calcimedin, Annexin 3, annexin A3, Annexin III, ANX3, ANXA3, Lipocortin III, PAP III



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, L02 cells, human heart tissue, human spleen tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66405-1-Ig targets Annexin A3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Annexin A3 fusion protein Ag2381
Full Name annexin A3
Calculated molecular weight 322 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000871
Gene symbol ANXA3
Gene ID (NCBI) 306
RRIDAB_2881779
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Protocols

Product Specific Protocols
WB protocol for Annexin A3 antibody 66405-1-IgDownload protocol
IHC protocol for Annexin A3 antibody 66405-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols