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Annexin A3 Monoclonal antibody (66405-1-Ig)

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WB analysis using 66405-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 66405-1-Ig
Caco-2 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 66405-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 66405-1-Ig
WB result of Annexin A3 antibody (66405-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A3 transfected Caco-2 cells.
WB analysis of HEK-293 using 66405-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 66405-1-Ig
L02 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 66405-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 66405-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 40x lens).

KD/KO Validated

Annexin A3 Monoclonal antibody

Annexin A3 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66405-1-Ig

Clone No.1A11E4

Publications(2)

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ANXA3, 1A11E4, 35 alpha calcimedin, 35-alpha calcimedin, Annexin 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HCT 116 cells, Caco-2 cells, HEK-293 cells, HepG2 cells, L02 cells, SW 1990 cells, DU 145 cells, SCaBER cells, HeLa cells, MCF-10A cells, human heart tissue, human spleen tissue
Positive IHC detected in	human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

66405-1-Ig targets Annexin A3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag2381 Product name: Recombinant human ANXA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-322 aa of BC000871 Sequence: MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGD Predict reactive species
Full Name	annexin A3
Calculated Molecular Weight	322 aa, 36 kDa
Observed Molecular Weight	32-36 kDa
GenBank Accession Number	BC000871
Gene Symbol	Annexin A3
Gene ID (NCBI)	306
RRID	AB_2881779
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P12429
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Protocols

Product Specific Protocols
IHC protocol for Annexin A3 antibody 66405-1-Ig	Download protocol
WB protocol for Annexin A3 antibody 66405-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Mar Drugs Sulfated Pelvetia siliquosa Polysaccharides Attenuate Pyroptosis via NF-κB Pathway Inhibition Against Calcium Oxalate Stone Formation. Authors - Xin-Yi Tong View Article
mouse	WB,IHC,IF	Oncogene Epstein-Barr virus causes vascular abnormalities in epithelial malignancies through upregulating ANXA3-HIF-1α-VEGF pathway Authors - Yuanyuan Chen KO Validated View Article