Annexin A3 Monoclonal antibody, PBS Only

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• Annexin A3 Monoclonal antibody, PBS Only (66405-1-PBS)

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  WB analysis using 66405-1-Ig (same clone as 66405-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 66405-1-Ig (same clone as 66405-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66405-1-Ig (same clone as 66405-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Caco-2 using 66405-1-Ig (same clone as 66405-1-PBS)

  WB result of Annexin A3 antibody (66405-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A3 transfected Caco-2 cells. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66405-1-Ig (same clone as 66405-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66405-1-Ig (same clone as 66405-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66405-1-Ig (same clone as 66405-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 66405-1-Ig (same clone as 66405-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

Annexin A3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66405-1-PBS

Clone No.1A11E4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ANXA3, 1A11E4, 35 alpha calcimedin, 35-alpha calcimedin, Annexin 3

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Product Information

66405-1-PBS targets Annexin A3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2381

  Product name: Recombinant human ANXA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-322 aa of BC000871

  Sequence: MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGD

• Full Name: annexin A3
• Calculated Molecular Weight: 322 aa, 36 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC000871
• Gene Symbol: Annexin A3
• Gene ID (NCBI): 306
• RRID: AB_2881779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12429
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.