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Annexin VI Polyclonal antibody, PBS Only (12542-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 12542-1-AP (same clone as 12542-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 12542-1-AP (same clone as 12542-1-PBS)
WB result of Annexin VI antibody (12542-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin VI transfected HeLa cells. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12542-1-AP (same clone as 12542-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 12542-1-AP (same clone as 12542-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 12542-1-AP (same clone as 12542-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 12542-1-AP (same clone as 12542-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
WB analysis of mouse placenta using 12542-1-AP (same clone as 12542-1-PBS)
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 12542-1-AP (same clone as 12542-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 12542-1-AP (same clone as 12542-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 12542-1-AP (same clone as 12542-1-PBS)
IP result of anti-Annexin VI (IP:12542-1-AP, 4ug; Detection:12542-1-AP 1:2000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IP experiment of mouse kidney using 12542-1-AP (same clone as 12542-1-PBS)
IP result of anti-Annexin VI (IP:12542-1-AP, 4ug; Detection:12542-1-AP 1:2000) with mouse kidney tissue lysate 3400 ug. This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 12542-1-AP (same clone as 12542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 12542-1-AP (same clone as 12542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 12542-1-AP (same clone as 12542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12542-1-AP (Annexin VI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 12542-1-AP (same clone as 12542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 12542-1-AP (same clone as 12542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 12542-1-AP (same clone as 12542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 12542-1-AP (same clone as 12542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 12542-1-AP (same clone as 12542-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Annexin VI antibody (12542-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12542-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Annexin VI Polyclonal antibody, PBS Only

Name: Annexin VI Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12542-1-PBS

Annexin VI Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12542-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Annexin A6, ANXA6, 67 kDa calelectrin, Annexin 6, Annexin-6

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12542-1-PBS targets Annexin VI in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3263 Product name: Recombinant human ANXA6 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 324-674 aa of BC017046 Sequence: DDDAAGQFFPEAAQVAYQMWELSAVARVELKGTVRPANDFNPDADAKALRKAMKGLGTDEDTIIDIITHRSNVQRQQIRQTFKSHFGRDLMTDLKSEISGDLARLILGLMMPPAHYDAKQLKKAMEGAGTDEKALIEILATRTNAEIRAINEAYKEDYHKSLEDALSSDTSGHFRRILISLATGHREEGGENLDQAREDAQVAAEILEIADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED Predict reactive species
Full Name	annexin A6
Calculated Molecular Weight	673 aa, 76 kDa
Observed Molecular Weight	67-72 kDa
GenBank Accession Number	BC017046
Gene Symbol	Annexin VI
Gene ID (NCBI)	309
RRID	AB_2057753
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08133
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.