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Arp5/ACTR5 Polyclonal antibody, PBS Only (21505-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse cerebellum using 21505-1-AP (same clone as 21505-1-PBS)
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)
WB result of Arp5/ACTR5 antibody (21505-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
WB analysis using 21505-1-AP (same clone as 21505-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 21505-1-AP (same clone as 21505-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
WB analysis of human skeletal muscle using 21505-1-AP (same clone as 21505-1-PBS)
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
WB analysis of mouse ovary using 21505-1-AP (same clone as 21505-1-PBS)
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)
IP result of anti-Arp5/ACTR5 (IP:21505-1-AP, 4ug; Detection:21505-1-AP 1:500) with HEK-293 cells lysate 1120ug. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 21505-1-AP (same clone as 21505-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 21505-1-AP (same clone as 21505-1-PBS)
Immunofluorescent analysis of A549 cells using 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 21505-1-AP (same clone as 21505-1-PBS)
Immunofluorescent analysis of HepG2 cells, using ACTR5 antibody 21505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Arp5/ACTR5 Polyclonal antibody, PBS Only

Name: Arp5/ACTR5 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 21505-1-PBS

Arp5/ACTR5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 21505-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ACTR5, ARP5, Actin related protein 5, Actin-related protein 5, hARP5

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

21505-1-PBS targets Arp5/ACTR5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15976 Product name: Recombinant human ACTR5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 258-607 aa of BC038402 Sequence: EELHKWRCPDYYENNVHKMQLPFSSKLLGSTLTSEEKQERLQQQLRRLQELNARRREEKLQLDQERLDRLLYVQELLEDGQMDQFHKALIELNMDSPEELQSYIQKLSIAVEQAKQKILQAEVNLEVDVVDSKPETPDLEQLEPSLEDVESMNDFDPLFSEETPGVEKPVTTVQPVFNLAAYHQLFVGTERIRAPEIIFQPSLIGEEQAGIAETLQYILDRYPKDVQEMLVQNVFLTGGNTMYPGMKARMEKELLEMRPFRSSFQVQLASNPVLDAWYGARDWALNHLDDNEVWITRKEYEEKGGEYLKEHCASNIYVPIRLPKQASRSSDAQASSKGSAAGGGGAGEQA Predict reactive species
Full Name	ARP5 actin-related protein 5 homolog (yeast)
Calculated Molecular Weight	607 aa, 68 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC038402
Gene Symbol	ARP5/ACTR5
Gene ID (NCBI)	79913
RRID	AB_10733470
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H9F9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)