p130Cas / BCAR1 Polyclonal antibody

p130Cas / BCAR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16815-1-AP

Synonyms

BCAR1, CRK associated substrate, CASS1, CAS1, CAS



Tested Applications

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, T-47D cells
Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16815-1-AP targets p130Cas / BCAR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p130Cas / BCAR1 fusion protein Ag10354
Full Name breast cancer anti-estrogen resistance 1
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2065460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IHC protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IF protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IP protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols