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CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal antibody

p130Cas / BCAR1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D11C10

Cat no : CL594-67215

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal (CL594-67215)

    IF Staining of human breast cancer using CL594-67215

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 p130Cas / BCAR1 antibody (CL594-67215, Clone: 2D11C10 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal (CL594-67215)

    IF Staining of human breast cancer using CL594-67215

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 p130Cas / BCAR1 antibody (CL594-67215, Clone: 2D11C10 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal (CL594-67215)

    FC experiment of A431 using CL594-67215

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human p130Cas / BCAR1 (CL594-67215, Clone:2D11C10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman breast cancer tissue
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67215 targets p130Cas / BCAR1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen p130Cas / BCAR1 fusion protein Ag10604
Full Name breast cancer anti-estrogen resistance 1
Calculated Molecular Weight 870 aa, 93 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2920097
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CL594 p130Cas / BCAR1 antibody CL594-67215Download protocol
FC protocol for CL594 p130Cas / BCAR1 antibody CL594-67215Download protocol
Standard Protocols
Click here to view our Standard Protocols