BCCIP Polyclonal antibody, PBS Only

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• BCCIP Polyclonal antibody, PBS Only (16043-1-PBS)

• 

  WB analysis of HeLa using 16043-1-AP (same clone as 16043-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 16043-1-AP (same clone as 16043-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16043-1-AP (same clone as 16043-1-PBS)

  IP result of anti-BCCIP (IP:16043-1-AP, 4ug; Detection:16043-1-AP 1:800) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16043-1-AP (same clone as 16043-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCCIP antibody (16043-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

BCCIP Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 16043-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

TOK1, TOK 1, Protein TOK-1, Protein TOK 1, P21- and CDK-associated protein 1

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Product Information

16043-1-PBS targets BCCIP in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8934

  Product name: Recombinant human BCCIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-314 aa of BC009771

  Sequence: MASRSKRRAVESGVPQPPDPPVQRDEEEEKEVENEDEDDDDSDKEKDEEDEVIDEEVNIEFEAYSLSDNDYDGIKKLLQQLFLKAPVNTAELTDLLIQQNHIGSVIKQTDVSEDSNDDMDEDEVFGFISLLNLTERKGTQCVEQIQELVLRFCEKNCEKSMVEQLDKFLNDTTKPVGLLLSERFINVPPQIALPMYQQLQKELAGAHRTNKPCGKCYFYLLISKTFVEAEKNNSKKKPSNKKKAALMFANAEEEFFYEKAILKFNYSVQEESDTCLGGKWSFDDVPMTPLRTVMLIPGDKMNEIMDKLKEYLSV

• Full Name: BRCA2 and CDKN1A interacting protein
• Calculated Molecular Weight: 314 aa, 36 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: BC009771
• Gene Symbol: BCCIP
• Gene ID (NCBI): 56647
• RRID: AB_2063712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P287
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.