BCL10 Monoclonal antibody

BCL10 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, FC

Conjugate

Unconjugated

CloneNo.

2E8G5

Cat no : 66556-1-Ig

Synonyms

B cell CLL/lymphoma 10, B cell lymphoma/leukemia 10, BCL10, c E10, CARD like apoptotic protein, CARMEN, cCARMEN, Cellular E10, Cellular homolog of vCARMEN, CIPER, CLAP, hCLAP, mE10



Tested Applications

Positive WB detected inHeLa cells, Raji cells, HepG2 cells, HCT 116 cells, MOLT-4 cells, Jurkat cells, K-562 cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:350-1:1400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66556-1-Ig targets BCL10 in WB, IHC, IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCL10 fusion protein Ag12162
Full Name B-cell CLL/lymphoma 10
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC053617
Gene symbol BCL10
Gene ID (NCBI) 8915
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BCL10 antibody 66556-1-IgDownload protocol
IHC protocol for BCL10 antibody 66556-1-IgDownload protocol
IF protocol for BCL10 antibody 66556-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

ZNF436 promotes tumor cell proliferation through transcriptional activation of BCL10 in glioma.

Authors - Yinwu Shang