CoraLite® Plus 488-conjugated BCL10 Monoclonal antibody

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  IF Staining of HepG2 using CL488-66556

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 BCL10 antibody (CL488-66556, Clone: 2E8G5 ) at dilution of 1:200, CL594-Phalloidin (red).

BCL10 Monoclonal Antibody for IF/ICC

Cat No. CL488-66556

Clone No.2E8G5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

B cell CLL/lymphoma 10, B cell lymphoma/leukemia 10, BCL10, c E10, CARD like apoptotic protein, CARMEN, cCARMEN, Cellular E10, Cellular homolog of vCARMEN, CIPER, CLAP, hCLAP, mE10

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66556 targets BCL10 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12162

  Product name: Recombinant human BCL10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-233 aa of BC053617

  Sequence: MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ

• Full Name: B-cell CLL/lymphoma 10
• Calculated Molecular Weight: 233 aa, 26 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC053617
• Gene Symbol: BCL10
• Gene ID (NCBI): 8915
• RRID: AB_3672915
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95999
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.