Tested Applications
Positive WB detected in | HeLa cells, K-562 cells, Raji cells, THP-1 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86183-1-RR targets BCL2L13 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | BCL2L13 fusion protein Ag9841 Predict reactive species |
Full Name | BCL2-like 13 (apoptosis facilitator) |
Calculated Molecular Weight | 485 aa, 53 kDa |
Observed Molecular Weight | 85 kDa |
GenBank Accession Number | BC003032 |
Gene Symbol | BCL2L13 |
Gene ID (NCBI) | 23786 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9BXK5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BCL2L13/MIL1 gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. BLC2L13 gene is highest expressed in heart, placenta and pancreas. The encoded protein Bcl-rambo may promote the activation of caspase-3 and apoptosis. The Bcl-rambo cell death activity was induced by its membrane-anchored C-terminal domain and not by the Bcl-2 homology region (PMID: 11262395). Multivariate analysis demonstrated that high expression of BCL2L13 was an independent prognostic factor for in childhood acute lymphoblastic leukemia (PMID: 20109966).
Protocols
Product Specific Protocols | |
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WB protocol for BCL2L13 antibody 86183-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |