BCL2L13 Recombinant antibody

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  WB analysis using 86183-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86183-1-RR (BCL2L13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86183-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86183-1-RR against Human BCL2L13 were performed. The affinity constant is 1.32 nM.

BCL2L13 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86183-1-RR

Clone No.250705F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Bcl2-L-13, Bcl-2-like protein 13, Bcl-rambo, CD003, MIL 1

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, Raji cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86183-1-RR targets BCL2L13 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCL2L13 fusion protein Ag9841
• Full Name: BCL2-like 13 (apoptosis facilitator)
• Calculated Molecular Weight: 485 aa, 53 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC003032
• Gene Symbol: BCL2L13
• Gene ID (NCBI): 23786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BXK5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L13/MIL1 gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. BLC2L13 gene is highest expressed in heart, placenta and pancreas. The encoded protein Bcl-rambo may promote the activation of caspase-3 and apoptosis. The Bcl-rambo cell death activity was induced by its membrane-anchored C-terminal domain and not by the Bcl-2 homology region (PMID: 11262395). Multivariate analysis demonstrated that high expression of BCL2L13 was an independent prognostic factor for in childhood acute lymphoblastic leukemia (PMID: 20109966).