Tested Applications
Positive WB detected in | pig liver tissue, HSC-T6 cells, HepG2 cells, HT-29 cells, COLO 320 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 4 publications below |
IHC | See 1 publications below |
Product Information
67448-1-Ig targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen |
CatNo: Ag7864 Product name: Recombinant human BDH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-343 aa of BC005844 Sequence: MLATRLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLGVKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHLPGAISDMIYIR Predict reactive species |
Full Name | 3-hydroxybutyrate dehydrogenase, type 1 |
Calculated Molecular Weight | 38 kDa |
Observed Molecular Weight | 31 kDa |
GenBank Accession Number | BC005844 |
Gene Symbol | BDH1 |
Gene ID (NCBI) | 622 |
RRID | AB_2882682 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q02338 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).
Protocols
Product Specific Protocols | |
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WB protocol for BDH1 antibody 67448-1-Ig | Download protocol |
IHC protocol for BDH1 antibody 67448-1-Ig | Download protocol |
IF protocol for BDH1 antibody 67448-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
MedComm (2020) BDH1-mediated LRRC31 regulation dependent on histone lysine β-hydroxybutyrylation to promote lung adenocarcinoma progression
| ||
bioRxiv An alternative route for β-hydroxybutyrate metabolism supports fatty acid synthesis in cancer cells
| ||
Acta Pharm Sin B Parabacteroides distasonis promotes liver regeneration by increasing β-hydroxybutyric acid (BHB) production and BHB-driven STAT3 signals | ||