BDH1 Monoclonal antibody

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  WB analysis of pig liver using 67448-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67448-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 67448-1-Ig

  WB result of BDH1 antibody (67448-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH1 transfected MCF-7 cells.

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  WB analysis of HepG2 using 67448-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67448-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 67448-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67448-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67448-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 67448-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse liver using 67448-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

BDH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67448-1-Ig

Clone No.2C3D4

Publications(4)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

2C3D4, 3-hydroxybutyrate dehydrogenase, BDH, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, EC:1.1.1.30

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Tested Applications

• Positive WB detected in: pig liver tissue, COLO 320 cells, HepG2 cells, HSC-T6 cells, HT-29 cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

67448-1-Ig targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7864

  Product name: Recombinant human BDH1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-343 aa of BC005844

  Sequence: MLATRLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLGVKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHLPGAISDMIYIR

• Full Name: 3-hydroxybutyrate dehydrogenase, type 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC005844
• Gene Symbol: BDH1
• Gene ID (NCBI): 622
• RRID: AB_2882682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q02338
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC	MedComm (2020); BDH1-mediated LRRC31 regulation dependent on histone lysine β-hydroxybutyrylation to promote lung adenocarcinoma progression; Authors - Jingjing Huang ; KD Validated
mouse,human	WB	bioRxiv; An alternative route for β-hydroxybutyrate metabolism supports fatty acid synthesis in cancer cells; Authors - Faith C Kaluba ; KD Validated
mouse	WB	Acta Pharm Sin B; Parabacteroides distasonis promotes liver regeneration by increasing β-hydroxybutyric acid (BHB) production and BHB-driven STAT3 signals; Authors - Manlan Guo
mouse	WB	PNAS Nexus; Frataxin controls ketone body metabolism through regulation of OXCT1; Authors - Yi NA Dong