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CoraLite®594-conjugated BID Monoclonal antibody

BID Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A2E5

Cat no : CL594-60301

Synonyms

BID, FP497, p11 BID, p22 BID



Tested Applications

Positive WB detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 60301-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

CL594-60301 targets BID in WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BID fusion protein Ag21005
Full Name BH3 interacting domain death agonist
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009197
Gene symbol BID
Gene ID (NCBI) 637
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).

Protocols

Product Specific Protocols
WB protocol for CL594 BID antibody CL594-60301Download protocol
Standard Protocols
Click here to view our Standard Protocols