BMP7 Polyclonal antibody, PBS Only

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• BMP7 Polyclonal antibody, PBS Only (12221-1-PBS)

• 

  WB analysis using 12221-1-AP (same clone as 12221-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12221-1-AP (BMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 12221-1-AP (same clone as 12221-1-PBS)

  WB result of BMP7 antibody (12221-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMP7 transfected HEK-293 cells. This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 12221-1-AP (same clone as 12221-1-PBS)

  IP result of anti-BMP7 (IP:12221-1-AP, 4ug; Detection:12221-1-AP 1:800) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12221-1-AP (same clone as 12221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12221-1-AP (same clone as 12221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 12221-1-AP (same clone as 12221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 12221-1-AP (same clone as 12221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bladder using 12221-1-AP (same clone as 12221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bladder using 12221-1-AP (same clone as 12221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12221-1-AP (same clone as 12221-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BMP7 antibody (12221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

BMP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12221-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

OP1, OP 1, Eptotermin alfa, Bone morphogenetic protein 7, BMP-7

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Product Information

12221-1-PBS targets BMP7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2902

  Product name: Recombinant human BMP7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 82-431 aa of BC008584

  Sequence: FMLDLYNAMAVEEGGGPGGQGFSYPYKAVFSTQGPPLASLQDSHFLTDADMVMSFVNLVEHDKEFFHPRYHHREFRFDLSKIPEGEAVTAAEFRIYKDYIRERFDNETFRISVYQVLQEHLGRESDLFLLDSRTLWASEEGWLVFDITATSNHWVVNPRHNLGLQLSVETLDGQSINPKLAGLIGRHGPQNKQPFMVAFFKATEVHFRSIRSTGSKQRSQNRSKTPKNQEALRMANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH

• Full Name: bone morphogenetic protein 7
• Calculated Molecular Weight: 431 aa, 49 kDa
• Observed Molecular Weight: 43-49 kDa
• GenBank Accession Number: BC008584
• Gene Symbol: BMP7
• Gene ID (NCBI): 655
• RRID: AB_2063960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18075
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).