CoraLite® Plus 488-conjugated BNIP3 Monoclonal antibody

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  IF Staining of HepG2 using CL488-68091

  Immunofluorescent analysis of (-20°C Methanol) fixed Cobalt Chloride treated HepG2 cells using CoraLite® Plus 488 BNIP3 antibody (CL488-68091, Clone: 2H7D8 ) at dilution of 1:200.

BNIP3 Monoclonal Antibody for IF/ICC

Cat No. CL488-68091

Clone No.2H7D8

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Synonyms

BNIP3, NIP3

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Tested Applications

• Positive IF/ICC detected in: Cobalt Chloride treated HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68091 targets BNIP3 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27935

  Product name: Recombinant human BNIP3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-194 aa of BC009342

  Sequence: MQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDDIERRKEVESILKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSAEFLKVFLPSLLLSHLLAIGLGIYIGRRLTTSTSTF

• Full Name: BCL2/adenovirus E1B 19kDa interacting protein 3
• Calculated Molecular Weight: 194 aa, 22 kDa
• Observed Molecular Weight: 25-30,50-60 kDa
• GenBank Accession Number: BC009342
• Gene Symbol: BNIP3
• Gene ID (NCBI): 664
• RRID: AB_3084396
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12983
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.