Beta Tubulin Monoclonal antibody, PBS Only

Beta Tubulin Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, nematode, pig, zebrafish

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D4A4

Cat no : 66240-1-PBS

Synonyms

beta tubulin, B-tubulin, MC1R, TUBB, Tubulin-beta

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Validation Data Gallery

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  WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

  IP result of anti-Beta Tubulin (IP:66240-1-Ig, 5ug; Detection:66240-1-Ig 1:1000) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66240-1-Ig (beta Tubulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66240-1-Ig (beta Tubulin antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66240-1-Ig (beta Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66240-1-Ig (same clone as 66240-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66240-1-Ig (beta Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66240-1-PBS targets Beta Tubulin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, nematode, pig, zebrafish samples.

• Tested Reactivity: human, mouse, rat, nematode, pig, zebrafish
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Beta Tubulin fusion protein Ag0117
• Full Name: tubulin, beta 3
• Calculated Molecular Weight: 450 aa, 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000748
• Gene Symbol: TUBB3
• Gene ID (NCBI): 10381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.