CoraLite® Plus 488-conjugated C-Peptide Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated C-Peptide Recombinant monoclonal antibody (CL488-87566-3)

C-Peptide Uni-rAb^® Recombinant Antibody for IF-P

Cat No. CL488-87566-3 Copied!

Clone No. 253514D7

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Synonyms

INS, insulin

• 

  IF Staining of human pancreas using CL488-87566-3

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human pancreas tissue using CoraLite® Plus 488 C-Peptide antibody (CL488-87566-3, Clone: 253514D7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human pancreas using CL488-87566-3

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human pancreas tissue using CoraLite® Plus 488 C-Peptide antibody (CL488-87566-3, Clone: 253514D7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF-P detected in: human pancreas tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-87566-3 targets C-Peptide in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: INS
• Calculated Molecular Weight: 110aa, 12 kDa
• GenBank Accession Number: NM_000207
• Gene Symbol: INS
• Gene ID (NCBI): 3630
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01308
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

C‑peptide is a 31‑amino‑acid peptide that connects the A and B chains of proinsulin and is co‑secreted with insulin in equimolar amounts from pancreatic beta cells. With a longer plasma half‑life than insulin, it serves as a reliable biomarker of endogenous insulin secretion. Clinically, measuring C‑peptide is crucial for distinguishing type 1 from type 2 diabetes, diagnosing hypoglycemia, and assessing residual beta‑cell function. Emerging evidence also suggests that C‑peptide may exert its own biological effects on vascular and renal tissues.