C-Reactive Protein/CRP Recombinant antibody

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  WB analysis of rat liver using 83741-3-RR

  rat liver tissue were subjected to SDS PAGE followed by western blot with 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 83741-3-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 83741-3-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83741-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83741-3-RR against Rat Crp were performed. The affinity constant is below 1 pM.

C-Reactive Protein/CRP {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 83741-3-RR

Clone No.240759B8

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, ELISA

Synonyms

PTX1, PTX 1, CRP, 240759B8

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Tested Applications

• Positive WB detected in: rat liver tissue
• Positive IHC detected in: rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83741-3-RR targets C-Reactive Protein/CRP in WB, IHC, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: C-reactive protein, pentraxin-related
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_017096.3
• Gene Symbol: Crp
• Gene ID (NCBI): 25419
• RRID: AB_3671339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P48199
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein belonging to the pentraxin family. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.