Filter: AllWBIHC

WB analysis using 67464-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 67464-1-Ig
WB result of C20orf3/APMAP antibody (67464-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C20orf3/APMAP transfected HepG2 cells.
WB analysis using 67464-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 67464-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis using 67464-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 67464-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 67464-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 67464-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 67464-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

C20orf3/APMAP Monoclonal antibody

Name: C20orf3/APMAP Monoclonal antibody
Brand: Proteintech
SKU: 67464-1-Ig

C20orf3/APMAP Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67464-1-Ig

Clone No.1C6C2

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, ELISA

Synonyms

C20orf3, Protein BSCv, BSCv, APMAP, Adipocyte plasma membrane-associated protein

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, HSC-T6 cells, rabbit liver tissue, HepG2 cells, LNCaP cells, NIH/3T3 cells, human placenta tissue, mouse liver tissue, rat liver tissue
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67464-1-Ig targets C20orf3/APMAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity	human, mouse, rat, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	C20orf3/APMAP fusion protein Ag29996 Predict reactive species
Full Name	chromosome 20 open reading frame 3
Calculated Molecular Weight	416 aa, 46 kDa
Observed Molecular Weight	42-46 kDa
GenBank Accession Number	BC003501
Gene Symbol	APMAP
Gene ID (NCBI)	57136
RRID	AB_2882693
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9HDC9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.