C8G Recombinant monoclonal antibody

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  WB analysis using 85652-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85652-1-RR (C8G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 85652-1-RR

  human plasma were subjected to SDS PAGE followed by western blot with 85652-1-RR (C8G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

C8G Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85652-1-RR

Clone No.242479G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C8C

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Tested Applications

• Positive WB detected in: human milk, human plasma, human placenta

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85652-1-RR targets C8G in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18261

  Product name: Recombinant human C8G protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-202 aa of BC113626

  Sequence: MLPPGTATLLTLLLAAGSLGQKPQRPRRPASPISTIQPKANFDAQQFAGTWLLVAVGSACRFLQEQGHRAEATTLHVAPQGTAMAVSTFRKLDGICWQVRQLYGDTGVLGRFLLQARGARGAVNVVVAETDYQSFAVLYLERAGQLSVKLYARSLPVSDSVLSGFEQRVQEAHLTEDQIFYFPKYGFCEAADQFHVLDEVRR

• Full Name: complement component 8, gamma polypeptide
• Calculated Molecular Weight: 202 aa, 22 kDa
• Observed Molecular Weight: 18-22 kDa
• GenBank Accession Number: BC113626
• Gene Symbol: C8G
• Gene ID (NCBI): 733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07360
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gamma chain of complement component 8 (C8G) is one of three different subunits (alpha, beta, and gamma) of the eighth complement component (C8), which is a component of the MAC (membrane attack complex) (PMID:33382892). Although the roles of C8A and C8B are directly associated with MAC formation, C8G is not essential in the assembly and activity of the MAC (PMID:11960635). Moreover, C8G was shown to inhibit neuroinflammation by interfering with the sphingosine-1-phosphate (S1P)-S1PR2 interaction (PMID:34149451).