CA2 Polyclonal antibody, PBS Only

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• CA2 Polyclonal antibody, PBS Only (16961-1-PBS)

• 

  WB analysis using 16961-1-AP (same clone as 16961-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 16961-1-AP (same clone as 16961-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human stomach using 16961-1-AP (same clone as 16961-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 16961-1-AP (same clone as 16961-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 16961-1-AP (same clone as 16961-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 16961-1-AP (same clone as 16961-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

  IP result of anti-CA2 (IP:16961-1-AP, 4ug; Detection:16961-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

CA2 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 16961-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Carbonate dehydratase II, Car2, CA-II, CAII, CAC

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Product Information

16961-1-PBS targets CA2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10680

  Product name: Recombinant human CA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-260 aa of BC011949

  Sequence: MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFAARGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK

• Full Name: carbonic anhydrase II
• Calculated Molecular Weight: 260 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC011949
• Gene Symbol: CA2
• Gene ID (NCBI): 760
• RRID: AB_2065857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00918
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3).