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Calbindin-D28k Polyclonal antibody
Calbindin-D28k Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 14479-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, human brain tissue, human kidney tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human cerebellum tissue, human small intestine tissue, human kidney tissue, human brain tissue, hippocampus|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse cerebellum tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14479-1-AP targets Calbindin-D28k in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calbindin-D28k fusion protein Ag5861|
|Full Name||calbindin 1, 28kDa|
|Calculated molecular weight||30 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC006478|
|Gene ID (NCBI)||793|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.
|Product Specific Protocols|
|WB protocol for Calbindin-D28k antibody 14479-1-AP||Download protocol|
|IHC protocol for Calbindin-D28k antibody 14479-1-AP||Download protocol|
|IF protocol for Calbindin-D28k antibody 14479-1-AP||Download protocol|
|IP protocol for Calbindin-D28k antibody 14479-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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