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  • KD/KO Validated

Calbindin-D28k Polyclonal antibody

Calbindin-D28k Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14479-1-AP

Synonyms

CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, D 28K



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, human kidney tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, human small intestine tissue, human kidney tissue, human brain tissue, hippocampus
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14479-1-AP targets Calbindin-D28k in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calbindin-D28k fusion protein Ag5861
Full Name calbindin 1, 28kDa
Calculated molecular weight 30 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC006478
Gene symbol CALB1
Gene ID (NCBI) 793
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Protocols

Product Specific Protocols
WB protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
IHC protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
IF protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
IP protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Death Dis

Differential effects of N-acetylcysteine on retinal degeneration in two mouse models of normal tension glaucoma.

Authors - Hiroki Sano
humanIF

Mol Med

Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.

Authors - Sha Li
mouseIF

Life Sci

Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy.

Authors - Qiwang Ma
mouseIF

Invest Ophthalmol Vis Sci

Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.

Authors - Kei Akaiwa
humanWB

Oncol Lett

Downregulation of calbindin 1, a calcium-binding protein, reduces the proliferation of osteosarcoma cells.

Authors - Zhengxiang Huang
  • KD Validated
mouseIF

Biochem Biophys Res Commun

Identification and immunohistochemical characterization of G-quadruplexes in mouse brain.

Authors - Sefan Asamitsu