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Calbindin-D28k Polyclonal antibody

Calbindin-D28k Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14479-1-AP


CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, Calbindin-D28k, D 28K

Tested Applications

Positive WB detected inmouse brain tissue, human kidney tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, hippocampus, human brain tissue, mouse cerebellum tissue, rat cerebellum tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cerebellum tissue, human kidney tissue, mouse kidney tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14479-1-AP targets Calbindin-D28k in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calbindin-D28k fusion protein Ag5861
Full Name calbindin 1, 28kDa
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC006478
Gene Symbol CALB1
Gene ID (NCBI) 793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.


Product Specific Protocols
WB protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
IHC protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
IF protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
IP protocol for Calbindin-D28k antibody 14479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Georgiana (Verified Customer) (09-11-2023)

Antibody labelled cell bodies and dendrites very clearly

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain
Calbindin-D28k Antibody Immunohistochemistry validation (1:1000 dilution) in mouse brain (Cat no:14479-1-AP)

Ramzi (Verified Customer) (06-14-2023)

The brain vibratome sections were treated with Acid-Alcohol before the blocking solution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: brain tissue ''Vibratome ''
Calbindin-D28k Antibody Immunofluorescence validation (1:300 dilution) in brain tissue ''Vibratome '' (Cat no:14479-1-AP)

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other