Caldesmon Polyclonal antibody

• Featured Product
• KD/KO Validated

Caldesmon Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20887-1-AP

Synonyms

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

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Validation Data Gallery

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  WB analysis of HeLa using 20887-1-AP

  WB result of Caldesmon antibody (20887-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caldesmon transfected HeLa cells.

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  WB analysis using 20887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20887-1-AP (Caldesmon antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20887-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20887-1-AP (Caldesmon antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20887-1-AP (Caldesmon antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 20887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Caldesmon antibody (20887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 20887-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Caldesmon (20887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: A2780 cells, HeLa cells, HT-1080 cells, MDA-MB-231 cells, PC-3 cells, SKOV-3 cells
• Positive IHC detected in: human colon tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue, HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20887-1-AP targets Caldesmon in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caldesmon fusion protein Ag13676
• Full Name: caldesmon 1
• Calculated molecular weight: 538 aa, 63 kDa
• Observed molecular weight: 70-80 kDa
• GenBank accession number: BC040354
• Gene symbol: CALD1
• Gene ID (NCBI): 800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caldesmon (CALD1), a protein found in the cytoskeleton, plays an important role in proliferation, apoptosis, cell motility, adhesion, receptor function, and second messenger pathways. Caldesmon is a substrate of cdc2 kinase and Erk1/2 MAPK, and phosphorylation by either of these kinases reverses the inhibitory effects of caldesmon. Caldesmon(CALD1) is a prognostic biomarker and correlated with bladder cancer(PMID: 34733993) and immune infiltrates in gastric cancers(PMID: 34189308).

Publications

Species	Application	Title
human	WB	J Am Heart Assoc; Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.; Authors - Jiaqi Tang
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
human	WB,IF	Front Physiol; PMID: 30108509; Authors - Iddrisu B Yabasin
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
human	WB	Cancers (Basel); AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma; Authors - Jiakang Zhang
human,mouse	WB,IHC	iScience; PKR deficiency delays vascular aging via inhibiting GSDMD-mediated endothelial cell hyperactivation; Authors - Zhouyangfan Peng