CARD9 Polyclonal antibody, PBS Only

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• CARD9 Polyclonal antibody, PBS Only (10669-1-PBS)

• 

  WB analysis of THP-1 using 10669-1-AP (same clone as 10669-1-PBS)

  WB result of CARD9 antibody (10669-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD9 transfected THP-1 cells. This data was developed using the same antibody clone with 10669-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 10669-1-AP (same clone as 10669-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10669-1-AP (CARD9 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10669-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 10669-1-AP (same clone as 10669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10669-1-AP (CARD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10669-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CARD9 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 10669-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Caspase recruitment domain-containing protein 9, hCARD9

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Product Information

10669-1-PBS targets CARD9 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1057

  Product name: Recombinant human CARD9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 193-492 aa of BC008877

  Sequence: SEEKGAALMRNRDLQLEIDQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYIQVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQMEEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLETLVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGPAGLPGIGAVC

• Full Name: caspase recruitment domain family, member 9
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 55-62 kDa
• GenBank Accession Number: BC008877
• Gene Symbol: CARD9
• Gene ID (NCBI): 64170
• RRID: AB_2228323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H257
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in spleen and regulate activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.