CoraLite®594-conjugated CBLB Monoclonal antibody

CBLB Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, pig, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D3A2

Cat no : CL594-66353

Synonyms

CBLB, DKFZp686J10223, DKFZp779A0729, DKFZp779F1443, FLJ36865, FLJ41152, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

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Validation Data Gallery

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  IF Staining of U2OS using CL594-66353

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®594 CBLB antibody (CL594-66353, Clone: 2D3A2 ) at dilution of 1:200.

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  IF Staining of RAW 264.7 using CL594-66353

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite®594 CBLB antibody (CL594-66353, Clone: 2D3A2 ) at dilution of 1:100. DAPI (blue).

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Tested Applications

• Positive IF detected in: RAW 264.7 cells, U2OS cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66353 targets CBLB in IF applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: CBLB fusion protein Ag18757
• Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Calculated Molecular Weight: 982 aa, 109 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC032851
• Gene Symbol: CBLB
• Gene ID (NCBI): 868
• RRID: AB_2919978
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.