CoraLite® Plus 647 Anti-Mouse CD103 (2E7)

CD103 Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2E7

Cat no : CL647-65047

Synonyms

A530055J10, alpha E1, alpha M290, aM290, CD103, Integrin alpha E, Itgae

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL647-65047

  1x10^6 mouse splenocytes were surface stained with 0.4 ug CoraLite® Plus 647 Anti-Mouse CD103 (CL647-65047, Clone:2E7) or 0.4 ug Isotype Control, and 0.4 ug CoraLite® Plus 488 Anti-Mouse CD3 (17A2) (CL488-65077, Clone: 17A2). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65047 targets CD103 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)
• Full Name: integrin alpha E, epithelial-associated
• GenBank Accession Number: BC150690
• Gene Symbol: CD103
• Gene ID (NCBI): 16407
• RRID: AB_2883652
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).