CoraLite® Plus 647 Anti-Mouse CD103 (2E7)

CD103 Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2E7

Cat no : CL647-65047

Synonyms

A530055J10, alpha E1, alpha M290, aM290, CD103, Itgae

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Validation Data Gallery

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  FC experiment of BALB/c mouse splenocytes using CL647-65047

  1X10^6 BALB/c mouse splenocytes were surface stained with 5 ul CoraLite®488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and CoraLite® Plus 647-conjugated Armenian Hamster IgG isotype control (left) or 5 ul CoraLite® Plus 647 Anti-Mouse CD103 (CL647-65047, Clone:2E7) (right). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65047 targets CD103 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)
• Full Name: integrin alpha E, epithelial-associated
• GenBank Accession Number: BC150690
• Gene Symbol: Itgae
• Gene ID (NCBI): 16407
• RRID: AB_2883652
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).