CoraLite®647 Anti-Mouse CD103 (2E7)

CD103 Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

2E7

Cat no : CL647-65047

Synonyms

A530055J10, alpha E1, alpha M290, aM290, CD103, Itgae



Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65047-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65047 targets CD103 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)
Full Name integrin alpha E, epithelial-associated
GenBank accession numberBC150690
Gene symbol Itgae
Gene ID (NCBI) 16407
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).

Protocols

Product Specific Protocols
FC protocol for CL647 CD103 antibody CL647-65047Download protocol
Standard Protocols
Click here to view our Standard Protocols