Anti-Human CD107b / LAMP2 (H4B4) Rabbit IgG Recombinant Antibody

• 

  FC experiment of human PBMCs using 65520-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-RR, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using 65520-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-RR, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using 65520-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-RR, Clone:H4B4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.2 ug Anti-Human CD107b Mouse Monoclonal Antibody (Clone:H4B4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

CD107b / LAMP2 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 65520-1-RR

Clone No.H4B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CD107b, LAMP2, CD107 antigen-like family member B, H4B4, LAMP 2

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65520-1-RR targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human adherent peripheral blood cells

• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC002965
• Gene Symbol: LAMP2
• Gene ID (NCBI): 3920
• ENSEMBL Gene ID: ENSG00000005893
• RRID: AB_3670306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P13473
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).