Anti-Human CD117 (104D2)

CD117 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1 kappa

Reactivity

Human, Bovine

Applications

FC

Conjugate

Unconjugated

CloneNo.

104D2

Cat no : 65154-1-Ig

Synonyms

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit



Tested Applications

Positive FC detected inTF-1 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65154-1-Ig targets CD117 in FC applications and shows reactivity with Human, Bovine samples.

Tested Reactivity Human, Bovine
Host / Isotype Mouse / Mouse IgG1 kappa
Class Monoclonal
Type Antibody
Immunogen MOLM-1 megakaryocytic cells
Full Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Calculated molecular weight 976 aa, 110 kDa
GenBank accession numberBC071593
Gene symbol KIT
Gene ID (NCBI) 3815
Conjugate Unconjugated
Form Liquid
Purification Method Purified by protein-A affinity chromatography
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Protocols

Product Specific Protocols
FC protocol for CD117 antibody 65154-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols