CoraLite®594 Anti-Mouse CD117/c-Kit Rabbit Recombinant Antibody

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  FC experiment of mouse bone marrow cells using CL594-98041

  1x10^6 mouse bone marrow cells were surface stained with CoraLite® Plus 488 Anti-Mouse CD45R, and 0.25 ug CoraLite®594 Anti-Mouse CD117/c-Kit Rabbit Recombinant Antibody (CL594-98041, Clone: 240246C11) or Isotype Control. Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL594-98041

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD117/c-Kit Rabbit Recombinant Antibody (CL594-98041, Clone: 240246C11) (red) or Isotype Control (blue). Cells were not fixed.

CD117/c-Kit Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL594-98041

Clone No.240246C11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD117, Kit, 240246C11, c KIT

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98041 targets CD117/c-Kit in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD117/c-Kit fusion protein Eg0883
• Full Name: kit oncogene
• Calculated Molecular Weight: 109 kDa
• GenBank Accession Number: NM_001122733.1
• Gene Symbol: CD117
• Gene ID (NCBI): 16590
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05532-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).