Anti-Rat CD11b/c (OX42) Mouse IgG2a Recombinant Antibody, PBS Only

• 

  FC experiment of rat bone marrow cells using 65606-1-MR (same clone as 65606-1-PBS)

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug Anti-Rat CD11b/c Mouse IgG2a Recombinant Antibody (65606-1-MR, Clone: OX42) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65606-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat bone marrow cells using 65606-1-MR (same clone as 65606-1-PBS)

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug Anti-Rat CD11b/c Mouse IgG2a Recombinant Antibody (65606-1-MR, Clone: OX42) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65606-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat bone marrow cells using 65606-1-MR (same clone as 65606-1-PBS)

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug Anti-Rat CD11b/c Mouse IgG2a Recombinant Antibody (65606-1-MR, Clone: OX42) (left) or 0.25 ug Anti-Rat CD11b/c (65229-1-Ig, Clone: OX42) (right) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65606-1-PBS in a different storage buffer formulation.

CD11b/c Uni-rAb^® Recombinant Antibody for FC

Cat No. 65606-1-PBS

Clone No.OX42

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

Synonyms

CD11b, integrin alpha M, Itgam, OX42

---

Product Information

65606-1-PBS targets CD11b/c in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: NM_012711
• Gene Symbol: CD11b
• Gene ID (NCBI): 25021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9JI30
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).