APC Anti-Human CD11b (ICRF44)
CD11b Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human and More (1)
Applications
FC
Conjugate
APC Fluorescent Dye
CloneNo.
ICRF44
Cat no : APC-65116
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human peripheral blood granulocytes |
Recommended dilution
Application | Dilution |
---|---|
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
FC | See 1 publications below |
Product Information
APC-65116 targets CD11b in FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Rheumatoid synovial cells and human monocytes |
Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
Calculated Molecular Weight | 1152 aa, 127 kDa |
GenBank Accession Number | BC096346 |
Gene Symbol | CD11b |
Gene ID (NCBI) | 3684 |
RRID | AB_2883004 |
Conjugate | APC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
Product Specific Protocols | |
---|---|
FC protocol for APC CD11b antibody APC-65116 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Discov Suppression of the hyaluronic acid pathway induces M1 macrophages polarization via STAT1 in glioblastoma. |